Genotyping

Genotyping facility – CIG common facility

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Mission

The main activity of this service is to screen (by specific PCR amplification) transgenic mise housed at the CIG animal facility.

Genotyping

The facility realises routine PCR with the use of a robot (Freedom EVO from Tecan) for series of 20 or more samples. P1000526.JPG
The robot performs pipeting manipulations for :
  • fast DNA preparation from ear biopsies (link to biopsy and extraction protocols)
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  • pipeting DNA and mastermix in PCR microplates
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  • loading agarose gels (E-gels de Invitrogen) or running on QIAxcel system
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Screens made in routine are available on request.

The facility is able to transfer manual screen to robot analyse (primers testing, DNA extraction, amplification parameters…) or to optimize a new screen (for example moving from Southern to PCR analysis, establishment of a new protocol…).

Results of the genotyping are entered by the facility into the Pyrat System database, co-managed with the animal facility.

An analyses request form should be joined with the samples. Links to the request form (en/fr):

Samples are stored 6 months in the laboratory after genotyping.

Price of analyses are fixed by the CIG direction and bills are established for each research group.

The facility is located in laboratory 2013 in the Génopode building (phone: +41 21 692 4136).

For any request please contact : cig_genotyping (at) unil.ch

The Staff

Function Name Phone
Head of the Facility Fabienne Lammers +41 21 692 4136
Lab Technician Katharina Hausherr +41 21 692 4136
Supervisor Nicole Vouilloz +41 21 692 3903
Scientific Advisor Professor Alexandre Reymond +41 21 692 3961
Génopode - CH-1015 Lausanne
Switzerland
Tel. +41 21 692 22 00
Fax +41 21 692 22 11