The insulin tolerance test (ITT) is commonly used in rodents to evaluate insulin sensitivity. The ability of the intraperitoneal insulin load to induce hypoglycemia is an index of insulin sensitivity. There are, however, several confounding factors including differential basal glucose between groups, counteracting effects of counterregulation, and effects of acute handling stress. Therefore, when alterations in insulin sensitivity (resistance or hypersensitivity) are suspected on the basis of an ITT, the gold standard, quantitative analysis is performed using the euglycemic, hyperinsulinemic clamp technique with tracers.
A minimum of 10 mice per group should be sent to our facility, one week prior to the experiment.
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Preitner F, Mody N, Graham TE, Peroni OD, Kahn BB. Long-term Fenretinide treatment prevents high-fat diet-induced obesity, insulin resistance, and hepatic steatosis. Am J Physiol Endocrinol Metab. 2009 Dec;297(6):E1420-9