- Routine determination of enzymatic activities, substrates and electrolytes concentrations in blood samples using the Cobas C111 diagnostic robot.
- Individual assays, or “profiles” for metabolites, tissue markers, etc.
- Given the obligatory “dead volume” required, the robot is best suited for multiple analyses on each sample.
Table 1. Panels of Analyses, volumes and sampling procedures.
Please contact Gilles.Willemin@unil.ch before collecting samples, at least 7 days in advance to discuss the blood sampling procedure.
After discussion, please fill in the PDF Analysis request form and send it by email. Bring the samples (in 1.7 ml Eppendorf tubes or individual 500 µl tubes) to Gilles Willemin in RN 2022.
Sample preparation should be performed as per specific assay requirements specified in Table 1.
Note: in addition to the total analysis volume (cumulated volumes for each analysis), please add a 30µl dead volume of plasma/serum. Sample remains will be available to users after completion of the analyses
Sample collection and preparation
For blood sampling good practices, see Biofluid Assays / Resources
Nature of the samples (to be discussed with us in advance)
Serum. Collect blood into tubes (with/out coagulation activators). Incubate at room temperature for 15 minutes (clotting). Centrifuge 10’, 4,500 rpm @ 4°C. Transfer supernatant into Eppendorf tubes of 0.5ml preferentially (no strips), or 1.5ml. Store according to Table 1.
Plasma. Collect blood into tubes with the appropriate anticoagulant at room temperature. Centrifuge 10’, 4,500 rpm. Transfer supernatant into Eppendorf tubes of 1.5ml (or 0.5ml but no strips). Store according to Table 1.
Sample volume must include analyses plus the dead volume (refer to Table 1). Please contact us for any question.
Sample labeling. Tubes should be clearly labeled with easy codes (e.g. numbers #1-100) using permanent markers. Please include a list with detailed information about the nature and the number of samples.
Strictly avoid hemolysis. Hemolysis can have a moderate to huge impact on the outcome of some specific tests listed in Table 1. Ways to avoid it include:
- Never shake or whip blood samples (inversion is ok)
- Never draw/eject too quickly blood from a capillary, needle, tip or syringe
- During tail bleeding, do not apply too much pressure on the tail.