Luciferase Test
Assay
- Thaw frozen stocks of biosensor cells and revive them in sterile luria broth
- Mix biosensor suspensions with the aqueous samples directly in luminometer titer plates
- Tape plates with Parafilm and incubate them in a rotary shaker at 30°C for at least 30 minutes
- Add a drop of decanal solution to the assay mixtures
- Mix and incubate for another 3 minutes
- Measure light emission by luminometry
Material
- biosensor strain Escherichia coli (pJAMA-arsR)
- luminometer
- luria broth
- arsenite stock solution
- thermomixer
- n-Decanal solution
Data handling
Arsenic concentrations in the samples are interpolated from a calibration curve.
