Cryopreservation and Rederivation Unit

Welcome to the Cryopreservation and Revitalisation Service of the Faculty of Biology and Medicine. Our mission is to support high-level scientific research in accordance with the 3R principles. We offer tailor-made solutions to help you with your projects, helping to reduce the number of animals needed to maintain strains through our cryopreservation services (link to the 3Rs page).

We offer a full range of services for murine lines.

Sperm Freezing

Sperm freezing is the ideal solution for securing a valuable strain, preventing genetic drift or conserving a strain that is no longer part of an active scientific study by archiving it. This makes it possible to maintain lines with no future project (application of the 3Rs) and to save on the cost of housing.

2 males (ideal age: 12 - 32 weeks) are needed to freeze 16 vials.

In Vitro Fertilisation (IVF) will be carried out as a quality control.

Our services include

sperm freezing
storage of straws.

Thawing and IVF

Our service maximises reproductive efficiency and reduces the time needed to revive lines of interest.

Our services include

Recovery of oocytes and sperm for in vitro fertilisation
Culturing embryos to a suitable stage for re-implantation
Assistance in creating new lines or revitalising existing lines.

Rederivation

Rederivation by embryo transfer allows line decontamination to eliminate potential pathogens. It is also necessary for the safe exchange of a line with external collaborators. Currently, rederivation takes place in a facility with a clean conventional status.

Our services include

The production of mice from cryopreserved embryos or sperm, or from mice housed in our quarantine facility
Transferring embryos into pseudopregnant females to generate pathogen-free mice
Support for the reintroduction of your lines in a controlled environment

Procedure

How to contact us? You can contact us using our form (here below) to discuss your specific requirements.
Analysis and proposal. We will analyse your needs and propose a personalised plan including appropriate services, timescales and estimated costs.
Implementation. Once the project has been validated, we will implement the agreed procedures, ensuring regular monitoring and keeping you informed at every stage of the process.

Cryo Contact Form.docx (15 Ko)

Fees

Our rates are competitive and tailored to the needs of academic and research institutions. Pricing information is available below. For a detailed cost estimate or to book our services, please contact us (email address and telephone number on the right of this page).

Cryo Fees.pdf (511 Ko)